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New microsatellite markers for garlic, Allium sativum (Alliaceae)

机译:大蒜,大蒜(蒜科)的新微卫星标记

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摘要

Premise of the study: A new set of microsatellite or simple sequence repeat (SSR) markers for garlic, an important medicinal spice, was developed to aid studies of genetic diversity and to define efficient strategies for germplasm conservation. Methods and Results: Using a (CT)(8)- and (GT)(8)-enriched library, a total of 16 SSR loci were developed and optimized in garlic. Ten loci were found to be polymorphic after screening 75 accessions. The parameters used to characterize the loci were observed and expected heterozygosity, number of alleles, Shannon Index, and polymorphism information content (PIC). A total of 44 alleles were identified, with an average of 4.4 alleles per loci. The vast majority of loci were moderate to highly informative according to PIC and the Shannon Index. Conclusion: The new SSR markers have the potential to be informative tools for genetic diversity, allele mining, mapping and associative studies, and in the management and conservation of garlic collections.
机译:研究的前提:开发了一套新的大蒜微卫星或简单序列重复(SSR)标记,这是一种重要的药用香料,可帮助研究遗传多样性并确定有效的种质保护策略。方法和结果:使用富含(CT)(8)-和(GT)(8)的文库,共开发了16个SSR基因座,并在大蒜中进行了优化。筛选75个种质后,发现10个基因座为多态性。观察到了用于表征基因座的参数,并预期了杂合性,等位基因数,Shannon指数和多态性信息含量(PIC)。总共鉴定出44个等位基因,每个基因座平均4.4个等位基因。根据事先知情同意和香农指数,绝大多数基因座是中度到高度信息化的。结论:新的SSR标记有可能成为遗传多样性,等位基因挖掘,作图和相关研究以及大蒜收集的管理和保存方面的信息工具。

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